ELISpot / FluoroSpot / ICS ELISpot (Enzyme-Linked ImmunoSpot) is a highly sensitive immunological assay that detects and quantifies secreted cytokines, chemokines, or other soluble proteins at the single-cell level. It is used to monitor immune responses by identifying cells that produce specific proteins in response to stimuli

ELISpot

ELISpot plays a crucial role in immunology, cancer research, vaccine development, and infectious disease studies. It provides a detailed understanding of how individual immune cells respond, offering insights into immune function and helping to evaluate the efficacy of vaccines and immunotherapies.

Accelevir’s ELISpot Services

Accelevir offers comprehensive ELISpot services, including:

• Custom Assay Design to target specific cytokines or immune responses.
• Single-Cell Analysis for highly sensitive detection of immune activity.
• High-Throughput Options to support larger studies and clinical trials.

Accelevir provides precise, high-sensitivity ELISpot services with customizable options for detecting immune responses, helping you make informed decisions in vaccine development and immunotherapy research.

FluoroSpot

FluoroSpot enhances immunological studies by enabling the simultaneous measurement of several immune markers in the same cell, providing a more comprehensive analysis of immune responses and improving the efficiency of research into complex immune reactions.

Accelevir’s FluoroSpot Services

Accelevir offers innovative FluoroSpot services, including:

• Multiplex Detection to identify several cytokines from the same cell.
• High Sensitivity for accurate immune monitoring.
• Customizable Panels to tailor assays to your specific research needs.

Accelevir’s FluoroSpot services offer the advantage of multiplex cytokine detection with high sensitivity, enabling deeper insights into immune responses for vaccine development and therapeutic research.

Intracellular Cytokine Staining (ICS) & Immune Cell Phenotyping

Comprehensive Immune Function Profiling for Translational and Clinical Research

Accelevir offers high-parameter Intracellular Cytokine Staining (ICS) and immune cell phenotyping to characterize functional immune responses in infectious disease, vaccine, cell therapy, and immuno-oncology studies. Our workflows integrate multiparameter flow cytometry with robust gating strategies to deliver reproducible, quantitative data for T cells, B cells, NK cells, myeloid cells, and other immune subsets.

ICS – Functional Immune Response Measurement

Intracellular Cytokine Staining enables the direct detection of cytokine production at the single-cell level following antigen-specific stimulation.

• Antigen-Specific T Cell Detection – Quantifying CD4⁺ and CD8⁺ T cells producing IFN-γ, TNF-α, IL-2, IL-4, IL-17, or other cytokines.
• Polyfunctionality Assessment – Measuring simultaneous cytokine expression to assess functional breadth of immune responses.
• Custom Stimulation Protocols – Peptide pools, viral antigens, tumor antigens, or novel immunogens.
• Low-Input Assay Optimization – Validated workflows for rare or precious PBMC samples from preclinical or clinical sources.

Immune Cell Phenotyping

Multiparameter immune phenotyping provides detailed characterization of immune cell populations, subsets, and activation status.

• Lineage & Subset Identification – CD3⁺ T cells, CD19⁺ B cells, CD56⁺ NK cells, monocytes, dendritic cells, and more.
• Activation & Exhaustion Markers – CD69, HLA-DR, PD-1, LAG-3, TIM-3, and other regulatory molecules.
• Memory & Differentiation Profiling – Naïve, central memory, effector memory, and terminal effector subsets.
• Therapeutic Impact Monitoring – Detecting immune modulation in response to vaccines, biologics, and cell therapies.

Applications Across Therapeutic Areas

• Vaccine Development – Measuring antigen-specific immune responses and polyfunctional T cell activity.
• HIV and Viral Pathogenesis – Profiling immune correlates of control, latency reversal, and therapeutic response.
• Cell Therapy – Tracking CAR-T, TCR, NK, and engineered immune cells in vivo.
• Immuno-Oncology – Evaluating tumor antigen-specific responses and immune checkpoint modulation.

Why Accelevir

• Multi-Color, High-Dimensional Panels – Optimized for up to 18+ parameters.
• Integrated Molecular + Immunology Workflows – Combine ICS/phenotyping with qPCR/dPCR or NGS from the same sample.
• Multi-Species Capability – Validated assays for human, NHP, and murine models.
• Regulatory Readiness – CLIA-certified and GLP-compliant workflows for pivotal studies.