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Digital PCR (dPCR) |
Our team has experience in developing, qualifying and scaling digital PCR workflows to support clinical trials. The superior performance of digital PCR is achieved via partitioning of each PCR reaction into thousands of nano-reactions, enabling direct multiplex interrogation and counting of individual target nucleic acid molecules. As such, digital PCR is uniquely adapted to the performance demands of current molecular assays across infectious disease and oncology. The platform is optimized for robust performance with a multitude of sample types, including blood, tissue, and cell-free nucleic acids.
Currently, our tests are broadly used to support HIV-1 clinical trials, and our growing portfolio includes proprietary and non-proprietary assays for pathogen quantification and human gene mutation detection.
Digital PCR (dPCR) represents a new frontier in quantitative molecular biology, providing researchers with unmatched accuracy for nucleic acid quantification. This advanced method allows for highly sensitive and precise measurements, particularly valuable in applications where detecting low-abundance targets or quantifying subtle variations is essential.
Accelevir Diagnostics is dedicated to offering robust Digital PCR (dPCR) services tailored to meet the demands of modern research, from oncology to infectious disease diagnostics. With dPCR, we empower you to achieve reliable, reproducible results that can drive impactful discoveries and informed decisions in your studies.
What is Digital PCR (dPCR)?
Digital PCR (dPCR) is a powerful new platform technology for detecting and quantifying DNA and RNA targets with higher precision, accuracy, and analytical sensitivity than can be achieved by traditional quantitative PCR (qPCR) approaches. Unlike qPCR, which relies on bulk template amplification and quantification relative to a standard curve, dPCR provides absolute quantification of DNA and RNA targets by relying on partitioning of each reaction into thousands of nanoliter-scale individual reactions. After endpoint thermal cycling, each of these dPCR partitions is individually scored as positive or negative for the template molecule of interest, and positive partitions are simply counted to produce an absolute quantity with no requirement for a reference standard curve.
dPCR is highly sensitive and can detect low-abundance targets, making it ideal for applications such as mutation detection, pathogen detection, gene expression analysis, and copy number variation analysis. Its robustness against PCR inhibitors and higher tolerance to target sequence variability further enhance its reliability and utility in both research studies and clinical trials.
Strengths of dPCR
Improved precision and accuracy: dPCR provides significantly higher precision and accuracy compared to qPCR, enabling researchers to demonstrate small changes in target copy number with confidence.
Superior analytical sensitivity: dPCR is highly sensitive and can detect very low-abundance targets, making it ideal for applications where detecting rare mutations or low-level pathogens is crucial. This sensitivity can be further enhanced using hyperwelling, allowing us to reliably detect one in a million targets in complex samples.
Resistance to PCR inhibitors: Unlike qPCR, dPCR is less affected by PCR inhibitors, which ensures reliable results even in challenging sample types.
Tunable tolerance to sequence variation: because dPCR relies on endpoint amplification, assays can be strategically designed to tolerate sequence variation at key positions without impacting quantitative accuracy.
Our capabilities
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Pathogen detection and quantification, including viruses, bacteria, and fungi
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Cell and gene therapy applications
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Lentivirus and AAV vector quantification
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Lentivirus and AAV vector integrity
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Biodistribution analysis
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Transgene expression
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Persistence
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Detection and quantification of gene editing events, including HDR and NHEJ
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Rare mutation and SNP detection
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Single-plex and multiplex assays for mutations of interest
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Linkage analysis for co-occurrence of multiple mutations on a single template molecule
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SNP discrimination assays
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Gene expression analysis
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Pre-designed assays available for humans, mice, and rats
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Single-plex and multiplex detection of mRNAs and microRNAs
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Copy number variation (CNV) analysis)
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Biodistribution analysis across tissues and organs
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Residual DNA quantification
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How we can support your project
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Custom assay design and optimization
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Analytical performance qualification
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Scale-up, including automation, to support high-throughput testing and large studies
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Providing dPCR and ddPCR testing services for clinical trials testing
Unlock the Precision of Digital PCR
Discover how Digital PCR can enhance your research precision with Accelevir’s specialized services. Contact us today to learn more about our dPCR solutions and how we can support your study with accurate, reliable, and reproducible data